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Summer course - basic in vitro

Basic in vitro laboratory practice & organ bath

Preliminary program

                                   (July 1-5, 2013 Price 1250€/person/course)

DAY 1

 

8.00-8.30

Arrival to the gate

8.30-8.45

Safety instructions at the gate

9.00-9.15

Welcome caffee&tea

9.15-10.15

Introduction; Labmagister; staff ,trainee;who is who; short program presentation;

10.15-10.30

Caffee break

10.30-12.30

Safety in the labs; sources of risks; self protecting tools; tools and realted risks;

12.30-13.30

Lunch break

13.30-17.00

Introduction of the of the most frequently used tools and equipment in the lab; pipets; syringes;stirres;heaters; lab balances; centrifuge etc.

DAY 2

 

8.00-8.30

Arrival to the gate

8.30-9.00

Welcome coffee&tea

9.00-10.00

Pipettes; which for what; how to use them

10.00-11.00

Laboratory balances; how to use; which for what and why; spatula

11.00-12.30

Dissolution;practicing the use both pipettes and balances

12.30-13.30

Lunch break

13.30-14.30

Laboratory centrifuge; which for what; safety

14.30-15.30

Measuring pH

15.30-16.30

Cleaning the lab; finishing the work; personnal hygenie

DAY 3

Organ bath assais 1

8.00-8.30

Arrival to the gate

8.30-9.00

Welcome coffee&tea

9.00-12.30

Theoretical background; organ bath; physiological solutions; systems; organs are using for;some important points-temperature-pH-O2/CO2 , types of agonis/antagonist examinations; pA2;pD2 etc.

12.30-13.30

Lunch break

13.30-17.00

Introduction of the equipment in the lab; preparation of the physiological solutions;

Short presentation of the data management software; calibration; call back old experiments

DAY 4

Organ bath assais 2

8.00-8.30

Arrival to the gate

8.30-9.00

Welcome coffee&tea

9.00-10.00

Final dissolutions-bath solution, agonist measurements etc.; execution of the animals-dissection of the organ; mounting organ;

10.00- 12.30

Lunch break

12.30-13.30

Agonist&wash out; Concentration-response; ED50 dose+antagonist+ED50 dose; final dissolutions-bath solution, agonist measurements etc.; execution of the animals-dissection of the organ; mounting organ;

DAY 5

Organ bath assais 3

8.00-8.30

Arrival to the gate

8.30-9.00

Welcome coffee&tea

9.00-10.00

Evaluation of the results; data treatment;-calculations-biostatistics;lab-notebook; formal requirements

10.00-12.30

Lunch break

12.30-13.30

Opportunity to drill what was learnt; free supervised excercise in the bench/laboratory.

 

Date: 
Monday, July 1, 2013 - 08:30 to Friday, July 5, 2013 - 13:30