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Summer course - advanced in vivo


Advanced in vivo laboratory practice

Lung inflammation&splenocyte culturing

Preliminary program

(July 15-19, 2013 Price 1250€/person/course)


DAY 1

 

8.00-8.30

Arrival to the gate

8.30-8.45

Safety instructions at the gate

9.00-9.15

Welcome caffee&tea

9.15-10.15

Introduction; Labmagister; staff ,trainee; who is who; short program presentation;

10.15-10.30

Caffee break

10.30-11.30

Making groups, tagging animals

12.30-13.30

Lunch break

13.30-14.30

Practicing intratracheal, intra nasal, evaporation challenges; preparation animals for Day 2.

14.30-17.00

Practicing bronchoalveolar lavage (BAL); cytocentrifusing; fixing smears; staining differentiation of leukocytes on control animals

DAY 2

 

8.00-8.30

Arrival to the gate

8.30-9.00

Welcome coffee&tea

9.00-12.30

Anaesthesia of rodent for BAL from Day 1 ; doing BAL; cytocentrifuging; staining of the smears; cell counting using hemocytometer

12.30-13.30

Lunch break

13.30-15.00

Leukocyte differentiation by light microscope, morphometry

15.00-17.00

Cell counting; registering in MS Excell skim/template;

DAY 3

 

8.00-8.30

Arrival to the gate

8.30-9.00

Welcome coffee&tea

9.00-12.30

Theoretical background; steril work; biosafety cabinet; self protection tools and clothes, pipetts are using during cell culture; CO2 thermostat

12.30-13.30

Lunch break

13.30-17.00

Introduction of the equipment in the lab; practising outside the biosafety cabinet; invert microscope; use of hemocytometer for cell counting, removal of splenocytes for culturing.

DAY 4

 

8.00-8.30

Arrival to the gate

8.30-9.00

Welcome coffee&tea

9.00-10.00

Practising what was learnt in Day 1&3 

10.00-12.30

LPS challenge of isolated splenocytes

12.30-13.30

Lunch break

13.30-17.00

Measuring one cytokine from the BAL by ELISA

DAY 5

 

8.00-8.30

Arrival to the gate

8.30-9.00

Welcome coffee&tea

9.00-2.30

Removal of splenocyte supernatant; preparation of the samples for ELISA measurements

12.30-13.30

Lunch break

13.30-17.00

Measuring one cytokine from the supernatant by ELISA

Date: 
Monday, July 15, 2013 - 08:30 to Friday, July 19, 2013 - 17:30