DAY 1
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8.00-8.30
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Arrival to the gate
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8.30-8.45
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Safety instructions at the gate
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9.00-9.15
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Welcome caffee&tea
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9.15-10.15
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Introduction; Labmagister; staff ,trainee; who is who; short program presentation;
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10.15-10.30
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Caffee break
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10.30-11.30
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On site presentation of Safety in the labs; sources of risks; self protecting tools; tools and realted risks;
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12.30-13.30
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Lunch break
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13.30-14.30
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Handling rodents
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14.30-17.00
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Treating rodents (i.v., i.p.,p.o., s.c., i.m., i.t., i.n. ); demonstration and practice; animal restraining
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DAY 2
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8.00-8.30
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Arrival to the gate
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8.30-9.00
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Welcome coffee&tea
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9.00-10.00
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Anaesthesia of rodents; why and how
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10.00-11.00
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Measuring and tagging rodents; making groups; different tagging methods and tools.
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11.00-12.30
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Trimming, shaving and epilation technics
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12.30-13.30
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Lunch break
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13.30-14.30
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Measuring body temperature
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14.30-15.30
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Aseptic interventions, aseptic workplace
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15.30-16.30
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Cleaning the lab; finishing the work; personnal hygenie
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DAY 3
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8.00-8.30
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Arrival to the gate
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8.30-9.00
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Welcome coffee&tea
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9.00-12.30
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Practicing intraperitoneal injection of proinflammatory trigger. (LPS or zymosan or eotaxin or …)
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12.30-13.30
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Lunch
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13.30-17.00
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Practicing peritoneal lavage cytocentrifusing; fixing smears; staining differentiation of leukocytes on control animals
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DAY 4
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8.00-8.30
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Arrival to the gate
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8.30-9.00
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Welcome coffee&tea
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9.00-12.30
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Intraperitoneal treatments of mice with pro-inflammatory trigger. Doing peritoneal lavage; cytocentrifuging; staining of the smears; cell counting using hemocytometer
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12.30-13.30
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Lunch break
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13.30-17.00
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Leukocyte differentiation by light microscope, morphometry; Cell counting; registering in MS Excell skim/template;
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DAY 5
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8.00-8.30
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Arrival to the gate
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8.30-9.00
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Welcome coffee&tea
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9.00-12.30
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Practising what was learnt in Day 1&4 using simple excercises,
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12.30-13.30
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Lunch break
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13.30-17.00
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Practising what was learnt in Day 1&4 using simple excercises;.
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