DAY 1
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8.00-8.30
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Arrival to the gate
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8.30-8.45
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Safety instructions at the gate
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9.00-9.15
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Welcome caffee&tea
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9.15-10.15
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Introduction; Labmagister; staff ,trainee; who is who; short program presentation;
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10.15-10.30
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Caffee break
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10.30-11.30
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Making groups, tagging animals
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12.30-13.30
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Lunch break
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13.30-14.30
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Practicing intratracheal, intra nasal, evaporation challenges; preparation animals for Day 2.
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14.30-17.00
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Practicing bronchoalveolar lavage (BAL); cytocentrifusing; fixing smears; staining differentiation of leukocytes on control animals
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DAY 2
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8.00-8.30
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Arrival to the gate
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8.30-9.00
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Welcome coffee&tea
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9.00-12.30
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Anaesthesia of rodent for BAL from Day 1 ; doing BAL; cytocentrifuging; staining of the smears; cell counting using hemocytometer
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12.30-13.30
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Lunch break
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13.30-15.00
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Leukocyte differentiation by light microscope, morphometry
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15.00-17.00
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Cell counting; registering in MS Excell skim/template;
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DAY 3
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8.00-8.30
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Arrival to the gate
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8.30-9.00
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Welcome coffee&tea
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9.00-12.30
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Theoretical background; steril work; biosafety cabinet; self protection tools and clothes, pipetts are using during cell culture; CO2 thermostat
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12.30-13.30
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Lunch break
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13.30-17.00
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Introduction of the equipment in the lab; practising outside the biosafety cabinet; invert microscope; use of hemocytometer for cell counting, removal of splenocytes for culturing.
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DAY 4
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8.00-8.30
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Arrival to the gate
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8.30-9.00
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Welcome coffee&tea
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9.00-10.00
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Practising what was learnt in Day 1&3
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10.00-12.30
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LPS challenge of isolated splenocytes
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12.30-13.30
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Lunch break
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13.30-17.00
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Measuring one cytokine from the BAL by ELISA
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DAY 5
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8.00-8.30
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Arrival to the gate
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8.30-9.00
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Welcome coffee&tea
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9.00-2.30
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Removal of splenocyte supernatant; preparation of the samples for ELISA measurements
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12.30-13.30
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Lunch break
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13.30-17.00
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Measuring one cytokine from the supernatant by ELISA
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