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Hands-on Organ Bath Techniques - Preliminary Detailed Programme

DAY 1

8.00-8.30

Arrival at the gate

8.30-8.45

Safety instructions at the gate

9.00-9.15

Welcome coffee & tea

9.15-10.15

Introduction; Labmagister; staff, trainee; who is who; short programme presentation; safety in the labs-gloves, glasses etc.; first aid

10.15-10.30

Coffee break

10.30-12.30

Theoretical background; organ bath; physiological solutions; organ is using for; some important points-temperature-pH-O2/CO2 , types of agonis/antagonist examinations; pA2;pD2 etc.

12.30-13.30

Lunch break

13.30-17.00

Introduction to the equipment in the lab; preparation of the physiological solutions;

Short presentation of the data management software; calibration; call back old experiments

DAY 2

8.00-8.30

Arrival at the gate

8.30-9.00

Welcome coffee & tea

9.00-12.30

Final dissolutions-bath solution, agonist measurements etc.; execution of the animals-dissection of the organ; mounting organ; spontaneous tone/without spontaneous tone

12.30-13.30

Lunch break

13.30-17.00

Agonist&wash out; Concentration-response; ED50 dose+antagonist+ED50 dose

Optional social programme at an additional cost

DAY 3

8.00-8.30

Arrival at the gate

8.30-9.00

Welcome coffee & tea

9.00-12.30

Evaluation of the results; data treatment;-calculations-biostatistics; lab-notebook; formal requirements

12.30-13.30

Lunch break

13.30-17.00

Opportunity to drill what was learnt; free supervised excercise in the bench/laboratory.

DAY 4

8.00-8.30

Arrival at the gate

8.30-9.00

Welcome coffee & tea

9.00-12.30

Free exercise

12.30-13.30

Lunch break

13.30-17.00

Free exercise

DAY 5

9.00-17.00

Option for one-to-one course for 350€/person

 

 

Optional social program for the weekend at an additional cost