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summer course - basic in vivo


Basic in vivo laboratory practice

Preliminary program

(July 8-12, 2013 Price 1250€/person/course)


DAY 1

 

8.00-8.30

Arrival to the gate

8.30-8.45

Safety instructions at the gate

9.00-9.15

Welcome caffee&tea

9.15-10.15

Introduction; Labmagister; staff ,trainee; who is who; short program presentation;

10.15-10.30

Caffee break

10.30-11.30

On site presentation of Safety in the labs; sources of risks; self protecting tools; tools and realted risks;

12.30-13.30

Lunch break

13.30-14.30

Handling rodents

14.30-17.00

Treating rodents (i.v., i.p.,p.o., s.c., i.m., i.t., i.n. ); demonstration and practice; animal restraining

DAY 2

 

8.00-8.30

Arrival to the gate

8.30-9.00

Welcome coffee&tea

9.00-10.00

Anaesthesia of rodents; why and how

10.00-11.00

Measuring and tagging rodents; making groups; different tagging methods and tools.

11.00-12.30

Trimming, shaving and epilation technics

12.30-13.30

Lunch break

13.30-14.30

Measuring body temperature

14.30-15.30

Aseptic interventions, aseptic workplace

15.30-16.30

Cleaning the lab; finishing the work; personnal hygenie

DAY 3

 

8.00-8.30

Arrival to the gate

8.30-9.00

Welcome coffee&tea

9.00-12.30

Practicing intraperitoneal injection of proinflammatory trigger. (LPS or zymosan or eotaxin or …)

12.30-13.30

Lunch

13.30-17.00

Practicing peritoneal lavage cytocentrifusing; fixing smears; staining differentiation of leukocytes on control animals

DAY 4

 

8.00-8.30

Arrival to the gate

8.30-9.00

Welcome coffee&tea

9.00-12.30

Intraperitoneal treatments of mice with pro-inflammatory trigger. Doing peritoneal lavage; cytocentrifuging; staining of the smears; cell counting using hemocytometer

12.30-13.30

Lunch break

13.30-17.00

Leukocyte differentiation by light microscope, morphometry; Cell counting; registering in MS Excell skim/template;

DAY 5

 

8.00-8.30

Arrival to the gate

8.30-9.00

Welcome coffee&tea

9.00-12.30

Practising what was learnt in Day 1&4 using simple excercises,

12.30-13.30

Lunch break

13.30-17.00

Practising what was learnt in Day 1&4 using simple excercises;.

 

Date: 
Monday, July 8, 2013 - 08:30 to Friday, July 12, 2013 - 17:00